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KMID : 0380020040190040245
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Korean Journal of Biotechnology and Bioengineering
2004 Volume.19 No. 4 p.245 ~ p.249
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Production of Yeast Extract by a Combined Method of Autolysis and Enzymatic Hydrolysis
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In Man-Jin
Chae Hee-Jung
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Abstract
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A combined method of autolysis and enzymatic hydrolysis of baker’s yeast was developed for the production of yeast extract, which is widely used as a natural food ingredient. From statistical analysis, NaCl and ethanol addition were found to be significantly effective factors in autolysis of yeast. The optimum dosages of salt and ethanol were 3% and 1%, respectively. Heat treatment and the use of cell lytic enzyme were not significantly effecting on the autolysis. Yeast hydrolysate was prepared by autolysis, followed by enzymatic hydrolysis using proteases, nuclease and deaminase. Additionally, the hydrolysate was processed by downstream process including Maillard reaction and debittering. The total dry matter yield and total nitrogen yield for the process were 76% and 59%, respectively. Compared to a process using brewer’s yeast, when baker’s yeast was used as a raw material, a higher recovery yield was obtained.
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KEYWORD
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Yeast extract, baker`s yeast, autolysis, enzymatic hydrolysis
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